in the news: September 2011

Friday, September 30, 2011

Amazon unveils Kindle Fire tablet, priced at $199 (Reuters)

NEW YORK (Reuters) ? Amazon.com Inc introduced its eagerly awaited tablet computer on Wednesday with a price tag that could make it the first strong competitor in a tablet market that has been dominated by Apple Inc's iPad.

The new device, priced at $199, may have the biggest impact on other makers of tablets and e-readers, such as Samsung Electronics Co Ltd and Barnes & Noble Inc, maker of the Nook.

"It's a Nook killer," said Scot Wingo, chief executive of ChannelAdvisor, which helps merchants sell more on websites including Amazon.com. "And it's a very compelling offering if you're not in the Apple ecosystem already."

The Kindle Fire tablet has a 7-inch screen, free data storage over the Internet and a new browser called Amazon Silk. Amazon expects shipments to start on November 15.

Amazon also introduced the Kindle Touch, an e-reader with no buttons and a touch screen starting at $99. And it cut the price of its basic Kindle e-reader to $79 from $99.

"These are premium products at non-premium prices," Chief Executive Jeff Bezos said. "We are going to sell millions of these."

Amazon shares rose 4.7 percent to $234.72 in midday trading, while Barnes & Noble dropped 9.8 percent to $11.92. Apple shares edged up 0.3 percent to $400.55.

Analysts had expected Amazon's tablet to be priced around $250, roughly half the price of Apple's dominant iPad, which starts at $499. The Nook Color e-reader costs $249.

"Expect a blood bath as pricing will have to get extremely aggressive," said Mark Gerber, an analyst at Detwiler Fenton & Co. He expects Amazon to sell at least 3 million Kindle Fires this holiday season, taking the No. 2 spot in the tablet market.

Having its own tablet is important for Amazon because the company has amassed a mountain of digital goods and services that could be sold through such a device.

The tablet might also encourage customers of Amazon, the world's largest Internet retailer, to shop online for physical products more often. (http://link.reuters.com/xab24s)

Breaking into the tablet market will be difficult. Companies including Hewlett Packard Co, Motorola Mobility Holdings Inc, Samsung and Research in Motion Ltd have launched tablets, but none has taken a big bite out of Apple's lead.

Apple dominates the North American tablet market, with 80 percent of the 7.5 million units shipped during the second quarter of 2011, according to Strategy Analytics.

(Additional reporting by Liana Balinsky-Baker in New York; Writing by Alistair Barr in San Francisco; Editing by Derek Caney and Gerald E. McCormick)

Source: http://us.rd.yahoo.com/dailynews/rss/business/*http%3A//news.yahoo.com/s/nm/20110928/bs_nm/us_amazon_tablet

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Defense says Jackson killed himself with drugs

LOS ANGELES?? First, prosecutors showed a photo of Michael Jackson's pale and lifeless body lying on a gurney. Then, they played a recording of his voice, just weeks before his death.

Slow and slurred, his words echoed Tuesday through a Los Angeles courtroom at the start of the trial of the doctor accused of killing him. As a worldwide audience watched on TV and Jackson's family looked on from inside the courtroom, a drugged Jackson said:

"We have to be phenomenal. When people leave this show, when people leave my show, I want them to say, 'I've never seen nothing like this in my life. Go. Go. I've never seen nothing like this. Go. It's amazing. He's the greatest entertainer in the world.'"

Video: Chilling audio, photos at trial of Jackson?s doctor (on this page)

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Prosecutors played the audio for the first time during opening statements as they portrayed Dr. Conrad Murray, 58, as an incompetent physician who used a dangerous anesthetic without adequate safeguards and whose neglect left the superstar abandoned as he lay dying.

Defense attorneys countered that Jackson caused his own death by taking a drug dose, including propofol, after Murray left the room.

Nothing the cardiologist could have done would have saved the King of Pop, defense attorney Ed Chernoff told jurors, because Jackson was desperate to regain his fame and needed rest to prepare for a series of crucial comeback concerts.

A number of Jackson's family members were in the courthouse, including his father Joseph, mother Katherine, sisters LaToya and Janet, and brothers Jermaine, Randy and Tito. LaToya Jackson carried a sunflower, her brother's favorite flower.

The family's most emotional moment came when the prosecutor played a video excerpt from Jackson's "This Is It" rehearsal in which he sang "Earth Song," a plea for better treatment of the environment.

As Jackson sang the words, "I used to dream. I used to glance beyond the stars," his mother, Katherine, dabbed at her eyes with a tissue.

Story: Cheat sheet for Michael Jackson doctor's trial

Prosecutor David Walgren noted it was Jackson's last performance.

Murray, who arrived at court holding hands with his mother, has pleaded not guilty to involuntary manslaughter. If convicted, he could face up to four years in prison and the loss of his medical license.

Speaking for more than an hour, Walgren relied on photos and audio recordings to paint Murray as an inept and reckless caretaker.

Walgren showed a photo of a lifeless Jackson on a hospital gurney. He juxtaposed the image with those of Jackson performing. Walgren also played the recording of Jackson speaking to Murray while, the prosecutor said, the singer was under the influence of an unknown substance roughly six weeks before his death.

Jackson trusted Murray as his physician, and "that misplaced trust in Conrad Murray cost Michael Jackson his life," Walgren said.

The recurring theme was Jackson's never-ending quest for sleep and propofol, the potion he called his "milk" and that he believed was the answer. Jurors were told that it was a powerful anesthetic, not a sleep aid, and the prosecutor said Murray severely misused it.

The prosecutor said while working for Jackson, the doctor was shipped more than four gallons of the anesthetic, which is normally given in hospital settings.

Story: Michael Jackson saw salvation in dangerous drug

Chernoff, the defense attorney, claimed the singer swallowed several pills of the sedative lorazepam on the morning of his death and that was enough to put six people to sleep. After taking a self-administered dose of propofol, Jackson did not even have a chance to close his eyes, Chernoff said, claiming he died instantly.

Chernoff, who had long hinted that the defense would blame Jackson for his own death, added a surprise. He claimed that Jackson died not because his doctor continued to give him the drug but because he stopped it, forcing Jackson to take extreme measures.

"What we will hear is that Dr. Murray provided propofol for two months to Michael Jackson for sleep," Chernoff said. "During those two months, Michael Jackson slept. He woke up and he lived his life.

"The evidence will not show you that Michael Jackson died because Dr. Murray gave him propofol. The evidence is going to show you Michael Jackson died when Dr. Murray stopped," the attorney said.

He said Murray was trying to wean Jackson off of propofol and had been giving him other sleep aids known as benzodiazepines trying to lull him to sleep.

On June 25, 2009, the last day of Jackson's life, Chernoff said, he was in the third day of a weaning process and it didn't work.

"Michael Jackson started begging. He couldn't understand why he wasn't sleeping.... When Michael Jackson told Dr. Murray 'I have to sleep. They will cancel my performance,' he meant it," Chernoff said.

Murray, in a recording of his interview with police detectives, acknowledged that he relented and agreed to give Jackson a small dose of propofol.

Walgren said Murray's claim that he gave the singer a minuscule dosage, enough to keep him asleep perhaps five minutes, was not true. He also accused Murray of deception when he hid from paramedics and hospital emergency staff that he had given Jackson propofol. He said they were desperately trying to revive him but didn't know about the drug.

He returned repeatedly to the fee Murray was to be paid ? $150,000 a month ? and pointed out that he first had asked for $5 million.

"There was no doctor-patient relationship," Walgren said. "... What existed here was an employer-employee relationship. He was not working for the health of Michael Jackson. Dr. Murray was working for a fee of $150,000."

Chernoff countered with a description of Murray's history of treating indigent patients for free. At times during the defense attorney's opening statements, Murray appeared to be crying and wiped his eyes with a tissue.

Jackson's family members appeared pained as Walgren described the singer as a vulnerable figure, left alone with drugs coursing through his body.

"It violates not only the standard of care but the decency of one human being to another," he said. "Dr. Murray abandoned Michael when he needed help."

Video: Reporter: Jackson?s ghost will haunt trial (on this page)

Following opening statements, Jackson's choreographer and friend, Kenny Ortega, testified that Jackson was in bad shape physically and mentally less than a week before his death.

He said he sent a message to Randy Phillips, producer of the "This Is It" concert, telling him that Jackson was ill, probably should have a psychological evaluation and was not ready to perform.

"It's important for everyone to know he really wants this," he wrote. "It would shatter him, break his heart if we pulled the plug. He's terribly frightened it's all going to go away."

In response to the email, Ortega said, a meeting was called at Jackson's house where Ortega clashed with Murray, who told him to stop playing amateur psychiatrist and doctor.

"He said Michael was physically and emotionally capable of handling all his responsibilities for the show,'" said Ortega, "I was shocked. Michael didn't seem to be physically or emotionally stable."

Within a few days, he said, Jackson had recouped his energy and was full of enthusiasm for the show.

During the defense opening statement, Chernoff referred to Dr. Arnold Klein, Jackson's dermatologist, who the judge decided will not testify.

The attorney tried to blame Klein for some of Jackson's woes, saying Klein gave Jackson the painkiller Demerol and he became addicted to it.

Slideshow: Michael Jackson?s life and career (on this page)

He told jurors that Klein would not be testifying but his records would be available and an addiction specialist would testify that one of the side effects of Demerol withdrawal is trouble sleeping. Chernoff said Murray was unaware of a Demerol shot administered to Jackson on June 16 and thus didn't realize there could be a fatal interaction with propofol.

Klein's attorney, Garo Ghazarian, later in the day issued a statement calling the allegations preposterous and "merely an attempt to whitewash the facts surrounding the death of ... Michael Jackson while under the management of Dr. Conrad Murray."

He noted there were no traces of Demerol in Jackson's autopsy or in his home, indicating he was not addicted. He also said Klein's use of the drug was not excessive. He noted that Klein was cleared by authorities of any wrongdoing in Jackson's death.

Dr. Conrad Murray could lose his medical license and get four years in prison if found guilty. Do you think that's fair? Discuss on Facebook. You can also follow our updates from the trial on Twitter @TODAY_ent.

Source: http://today.msnbc.msn.com/id/44684583/ns/today-entertainment/

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Thursday, September 29, 2011

Ideas to Help Prevent Blushing In Public ? Beauty, Health and Fitness

Blushing is an awkward problem. It never appears at the right time (not that there often is a ?right time? to turn an odd shade of red) but blushing in public is even worse. You feel that everyone around you has instantly noticed you change color and is staring at you. Which makes the problem even worse. So what can you do to prevent blushing in public or ? at the very least ? reduce the frequency that your face turns red?

Learn to relax

Quite often when we blush it?s the result of stresses and strains elsewhere in our life literally coming to the surface.

Relaxing is an art that we?ve mostly forgotten as we?ve grown older. So we actually have to re-learn it.

Short term, start by taking a few deep breaths when you feel that a blush is about to take over. This will generally help your body to relax and will reduce the tension you?re feeling.

Longer term, look into one of the many different relaxation techniques that are around. Have a play with them to see which works best for you. A lot of people use music to help them unwind. Even teenagers find the random white noise that passes for music as relaxation ? go figure!

Other things such as yoga or meditation or hypnosis are also worth investigating as methods to help you to relax more often.

Splash cold water on your face

Not exactly a high-tech solution. But if you?re able to excuse yourself for a minute or so, finding a cold tap and splashing cold water over your face will reduce the heat you?re feeling and can help to get your blushes a bit more under control.

Realize that not everyone is looking at you

Oftentimes we feel that the whole world is staring at us at the first sign that we?re starting to blush.

Which is miles away from the truth.

Most people are more pre-occupied with their own worries than they are to be worried about you. Which is why they often don?t notice even glaring problems like a bad hair day, let along your face changing to a deeper shade of red.

Our own self-consciousness is often the biggest thing to notice that you?ve even blushed at all.

So chill out a bit and don?t let the thought that other people might be looking at your embarrassment bug you. There?s a very high chance they haven?t even noticed unless you?ve drawn their attention to your problem.

Rehearse

OK, there?ll probably still be occasions where your mind goes blank. But if you can spend a bit of time rehearsing how your next public appearance will pan out, that?s a good way to reduce the chance of blushing taking over the whole proceedings.

Top athletes do this kind of thing all the time but the benefits aren?t just reserved for them.

Plan out what?s going to happen in a decent amount of detail. Without letting yourself get bugged if things don?t go totally to plan.

If you?re going to talk, run it through in your head first. Or talk out loud ? most people will think you?re on the phone nowadays.

Practice will help you get through whatever situation normally causes you to start blushing and at the very least will help reduce the problem even if it doesn?t prevent your blushing completely.

Source: http://www.akcesoria-kosmetyczne.pl/beauty-news/ideas-to-help-prevent-blushing-in-public-2/

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Online E Learning Computer Training Courses | plakatdruck ...

September 27th, 2011 by plakatdruck-hamburg.info Leave a reply ?

Have great reservations about some of the courses have been credited with strengthening design and interactive tools. High-paying jobs in computer programming anywhere in the world, if you do not feel that a useful task of learning new skills. First, the use of computers requires a lot of time and effort to Learning Technologies. This does not mean that it is a physical job, but a spirit of a lot of work. But you know, you really do not need college degree, is a skilled computer programmer? Yes, you only need to dedicated study and implementation of technology is not mature enough to learn programming. The flexibility you need to learn the time and place you want! Make sure your flexibility! This may be you can learn the 4 weeks, if put in enough computer programming.

Not everyone knows that computer programming, not everyone interested in learning these subjects. Why? If you want to learn how to Learning Software fast, you really need to consider the French learning software. Computer work drawback is that you cannot really work outside, tired eyes, some days, I cannot close my computer. In saying that I love to use computers. It is better if it is managed. So, you can log in from anywhere to use your own scheduling software. I find it hard, sacrifice their own learning, no matter what age, but I believe that anyone can learn the proper motivation to do anything, and started in the world, one of the fastest growing industries, a new career. Some well-known courses have very strict permission.

If you are such a specialized field of study, you will find very interesting Computer Training Courses, rather than boring, boring work is very useful. For example, you can only load them on a single computer, so if you have a laptop and desktop computers, you cannot use both them. , Forget the iPod or MP3 player, in this case! You can experts from around the world and learn what you need to know the paid programming. Computer programming is through the use of one or some computer programming language second language is this written in such a way, they understand the human, the machine also is not precise enough to understand. I first looked at the computer code, I was totally confused language, but I learned a computer language and practice of the code faster.

Source: http://plakatdruck-hamburg.info/2011/09/online-e-learning-computer-training-courses/

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Summarize Some Outstanding Moncler Jackets In 2011

September 27th, 2011 . by admin

For the people who want to be beautiful, a comfortable light Moncler Jackets is the product of essential in winter. Moncler Vest Men is the necessary sheet is tasted winter, temperatures in the time can always bring us warm, gentle feather won?t give a person too heavy impression, the seams of obvious for simple design add some design feeling, long version to clipping more for you in the whole winter increase warm feeling. Moncler Boots is all hands some for the winter sheet is tasted, don?t old land thought is the pronoun of down mast bloated, inside wear cool and refreshing, outside wear a down jacket, so you can walking in the rain.

We had summarized some outstanding Moncler Jackets in 2011; you can quickly keep up with the fashion trend. Down jacket over fat can make clothing and human body between air flow layer, increase the temperature heat faster; Thin will make the gaps in the down too small, containing smaller still air, which can reduce the warmth retention property.

Don?t think hypertrophy clothes will only let a person look bloated, actually otherwise, on the small but can highlight the legs under the slender, buttock and thick ham can cover off. Blue down long we will let you comfortable in winter. The long Moncler outlet Men inside build style is very good choice, is almost a joker. The business requirements and concise are travel to be more comfortable and convenient. Wear long chain or leather pearl for scarf elegant temperament finish show undoubtedly.This article is posted by velevens(110927).

Posted in Shopping and Product Reviews | Comments Off

Source: http://www.sweetcronthemes.com/shopping-and-product-reviews/summarize-some-outstanding-moncler-jackets-in-2011/

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Standardized Tests ? ??????????????? Has The Best ...

The faculty staffs of the landmark education are much dedicated and determined to train the students in order to make them achieve their dreams and goals. The faculty members also conduct personality development courses for the students, since education alone is not the life. There is something beyond the education which is character building. The landmark forum suggests so many types of personality development courses for its students worldwide so that they can be very accurate not only in their office work but also in their day to day life. This landmark forum also started providing personal performance coaching to their graduates to develop their life style. Some of them were navigating our lives: what really matters, inventing new futures; altering old, limiting realities, fighting aids in Africa, and a tree grows in Ethiopia. There are many landmark forums being conducted all around the world in many different countries.They provide more of a transformative learning rather the informative teaching. The recent survey has said that about 94% of people who underwent classes in landmark education have major difference in the way they live their lives. And also about 175,000 members are attending landmark forum every year. They teach people about how to leave. The informative learning will add additive information about what people knows and thereby extends our already known capacities. Whereas the transformative learning will provides the basic knowledge about how a human can survive in this competition filled environment. In this landmark forum, we can get answers for all our questions related to our life. To be precise, the landmark education center is present in about 53 locations around 19 countries.Usually there are three sessions being conducted for the students there for about three days. Some people will be thinking about climbing the corporate ladder in their work, but if they want to climb the ladder in their life, ??????????????? is the paradise.

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Wednesday, September 28, 2011

U.S. on "knife edge" of contraction: Fed economist (Reuters)

SAN ANTONIO (Reuters) ? The U.S. economy is on a "knife edge" between growth and contraction, and if it were a dashboard, it would be flashing "watch out, danger ahead on all gauges," Dallas Federal Reserve Bank's top economist said on Tuesday.

"The economy is moving along at stall speed," Dallas Fed research director Harvey Rosenblum told a forum sponsored by the greater San Antonio Chamber of Commerce. "Unless we start moving a little bit faster, we are at a tipping point where things may not go the right way."

The U.S. jobs engine has lost momentum and could be set for further "backtracking," Meanwhile, he said, there is also a "credible" risk of rising inflation.

"We are in the midst of the Second Great Contraction," Rosenblum said, demonstrating the economy's predicament with a picture of a place on the Appalachian Trail known as "Knife's Edge."

"This patient is still not ready to get out of the hospital, there are still tubes connected to the patient, and the patient is still not responding well to all the medicine."

The grim assessment of the economic outlook came a week after a majority of the Fed's policy-setting panel backed further monetary policy easing to help support a faltering U.S. recovery.

Dallas Fed President Richard Fisher, Rosenblum's boss, was one of three policymakers to dissent. Fisher gave a vigorous defense of his decision at his bank headquarters on Tuesday, saying he believed the move could do more harm than good.

In San Antonio, Rosenblum said that there is a better than even chance the United States will return to more than 2 percent growth, rather than fall into recession.

But at the recent policy-setting Federal Open Market Committee meeting, given a choice between whether the economy will be better than expected or worse than expected in the near future, most participants said it would be worse than expected, according to Rosenblum.

"The takeaway on the national economy is that economic growth has slowed and maybe stalled," he said. "We are on an edge."

(Reporting by Jim Forsyth in San Antonio; Writing by Ann Saphir in Dallas; Editing by James Dalgleish)

Source: http://us.rd.yahoo.com/dailynews/rss/business/*http%3A//news.yahoo.com/s/nm/20110927/bs_nm/us_usa_fed_contraction

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Pankaj Advani joins kids' movement

Bangalore, Sep 28

World billiards champion Pankaj Advani is auctioning his time during the 'Joy of Giving Week' to raise funds for Bangalore based NGO Children's Movement for Civic Awareness (CMCA).

CMCA is an 11-year old volunteer driven, civil society organisation that conducts a national 'civic club' programme in schools across India. Over the past year, CMCA has, through 284 volunteers, reached 19,000 children in six cities, 324 schools and 399 civic clubs.

Speaking about his association with the NGO, Advani said: ?For the democratic set-up to function effectively, it is essential for individuals to be aware of their rights, duties and responsibilities. CMCA works towards educating and encouraging the citizens of tomorrow to make a positive impact on the society that they live in.?

The NGO believes that Advani is its perfect goodwill ambassador, as his success inspires the youth of the nation to believe that with hard work and determination nothing is beyond reach.

Expressing joy at Advani's efforts to help them, CMCA's National Coordinator and Managing Trustee Vrunda Bhaskar, said: ?We are happy that young icons and role models like Pankaj Advani are committing themselves to a very important aspect of India's future as a secular and democratic nation - preparing our young to be active and empowered citizens who know how to stand up for their rights and strengthen our democracy.?

During the 'Joy of Giving Week', fans will have a chance to bid to spend time with Advani and contribute to a good cause.

The bidding for a chance to spend time with Pankaj will begin on eBay Friday and closes Oct 20, and the highest bidder will win a lunch and bowling session for two with the billiards icon and all the proceeds from this will go to CMCA.

Source: http://www.prokerala.com/news/articles/a250103.html

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Adult western birthday party invitations - Adult Birthday Party- Have ...

Adult Birthday Party- Have Fun With Adult Parties

Adult Birthday Party- Have Fun With Adult Parties

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Home Page > News and Society > Adult Birthday Party- Have Fun With Adult Parties

Adult Birthday Party- Have Fun With Adult Parties

Posted: Mar 26, 2010 |Comments: 0
| Views: 102 |

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Celebrating birthday has special significance, as it is the day when we receive cherished blessings from our near ones and dear ones in a grand way which are generally accompanied with hosting adult birthday party so as to feel the utmost excitements and joy as well. If you have an upcoming birthday or you are setting plans so as to celebrate your birthday in a special way which will become a source for, to derive fun and enjoyments for the rest of your life then the foremost thing you can opt for, is to host an birthday party in any clubs, or you can even hire a hall with all basic hospitalities required for, to feel the enthrallments associated with the celebrations. If you are planning to host a party accompanied with liquors then you should opt for any alternatives so as to make your children feel comfortable in such an atmosphere because you cannot host a party without your children. So it is your responsibility to find for any alternatives which will not deteriorate the level of fun and at the same time you are able to derive fun and enjoyments along with your family comprised of children.

During warmer season the parties are generally hosted outside in the open surrounding so as to feel the calm and serenity associated with nature. If your house is not big enough so as to accommodate for a party surrounding then you can opt for any barbeque or party hall which are implied with huge surrounding area so as to feel the excitements accompanied with such adult birthday party in the perfect way. For holding an outdoor party it is your concern to arrange it in accordance to the condition of the weather as rain might ruin your arrangements.

You can opt for any theme so as to decorate the ambience for your birthday celebration. It is of great concern that the party must appear grand and elaborate and it is completely different from those celebrating their kid's birthday or even celebrating someone's birthday that might be 70 or even 80 years of age. Adult party means the ambience should appear special in terms of arrangements and foods as well. You can invent gambling themes to your birthday ideas party celebration.

Owing to the western influence people has now become quite familiar with the casino games which are not only considered to be an important way of making money but also it helps in adding a sophisticated look to the party hosted by you. In order to earn for your friends appreciation for holding a unique party then you should implement this particular theme to your birthday party celebration so as to make it appear even more sophisticated and grand. As birthday are considered to be the most memorable event for each one us and so it is our duty to make it appear special for the people whom you are inviting for to celebrate your birthday accompanied with unique hospitality essential for an adult birthday party.

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It's always fun being a little naughty as you grow older, have fun with some Adult Birthday Ideas , and don't forget to check out some Free birthday invitations to add to the magic.

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Tuesday, September 27, 2011

Research reveals how dynamic changes in methylation can determine cell fate

Public release date: 26-Sep-2011
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Contact: Peter Tarr
tarr@cshl.edu
516-367-8455
Cold Spring Harbor Laboratory

Cold Spring Harbor, NY Scientists at Cold Spring Harbor Laboratory (CSHL) and the University of Southern California (USC) have uncovered intriguing new evidence helping to explain one of the ways in which a stem cell's fate can be determined.

The new data show how the "marking" of DNA sequences by groups of methyl molecules a process called methylation can influence the type of cell a stem cell will become. The cellular maturation process, called differentiation, has long been thought to be affected by methylation. Subtle changes in methylation patterns within subsets of a particular cell type have now been observed and closely scrutinized, and they reveal some intriguing mechanisms at work in the process.

A team led by postdoc Dr. Emily Hodges, working in the laboratory of CSHL Professor and HHMI Investigator Gregory Hannon, studied how methylation changes in blood stem cells can affect whether a given stem cell will differentiate into either a myeloid cell or a lymphoid cell. These are the two major lineages of mature blood cells. Sophisticated mathematical analyses of the data were performed under the direction of USC Professor Andrew D. Smith.

The study, which will appear in print October 7 in the journal Molecular Cell, generated some surprising findings that challenge currently held theories about how methylation operates. First, it demonstrated that methylation patterns are more dynamic than they are often thought to be. "It's not a question of methylation being 'on' or 'off' at a given site in the genome," explains Hodges. "We find, instead, an interesting fluctuation of the boundaries of regions that are free of methylation marks. This fact, in turn, can have a profound impact upon cell fate."

Areas lacking methylation, called hypomethylated regions, or HMRs, tend to coincide with so-called CpG islands, sites in the genome where adjacent "Cs" and "G's" cytosine and guanine nucleotides are seen in strings of repeats. These unmethylated regions tend to be ones associated with nearby genes that are capable of being expressed. In contrast, sites in the genome that are methylated are typically not expressed.

The new study, which looks at these areas at high resolution in cells of the different blood cell lineages and in blood stem cells, finds that in many cases, a core portion of the unmethylated region is shared in common, but that adjacent areas, sometimes called "CpG shores" the outlying areas around CpG islands differ markedly in breadth. The CSHL-USC team refines the notion of islands and shores, preferring to describe the narrowing and widening of the "shoreline" as a tidal phenomenon.

"We observed that the boundaries of these unmethylated regions goes in and out, like the tides," says Hodges. "The key question is what drives these changes. We found that the width of these regions depends on the gene that is associated with the region. We showed in blood cells that the variation is lineage-specific."

The team deduced this after making close study of the methylation patterns in genomic regions containing genes known from other research to be expressed specifically in lymphoid cells, but not in myeloid cells, or vice versa. In these cases, all blood cells share a narrow "core" region of hypomethylation; but only in one lineage did the unmethylated region widen a widening that opens the promoter of the "underlying" gene to the cellular machinery initiating gene expression. In other words, the lack of methylation over a wider area enables the underlying gene to be activated only in the specified cell-type, but not in any of the others.

Another striking observation made from this data is the directional preference of this expansion. For example, in the widening of the unmethylated region seen in the case of the lymphoid cell, the direction of the widening was toward the area occupied by the underlying gene, which in this case was a gene encoding a B cell surface marker called CD22.

It has generally been thought that methylation is a stable epigenetic mark and that changes in methylation are unidirectional; and further, that cells become increasingly methylated as they move through the differentiation process toward their mature identity. And in fact, the only known direction of active change is from an unmethylated state to a methylated state.

The new data suggests, however, that dynamic changes in methylation status may be possible. The relevant evidence comes from blood stem cells, which were observed to have methylation patterns that the team describes as "intermediately methylated," seemingly in dynamic equilibria of the two extreme states of "methylated" and "unmethylated."

According to Hodges, this raises the possibility that methylation might in fact be bidirectional, and that there might be an as yet undiscovered, active mechanism that performs de-methylation. No known enzyme has this ability to remove methyl groups from DNA; DNA methyltransferase is the well-known enzyme that catalyzes the addition of methyl groups.

Yet another of the team's unexpected findings concerns the position of HMRs relative to know genic regions. While unmethylated regions tend to be associated with nearby genes that are capable of being expressed, the team found, according to Hodges, "a lot of HMRs located far away from any annotated gene locus." One notable thing about these regions, she says, "is that they were highly enriched for binding sites of specific regulatory molecules that are involved in chromatin organization."

Chromatin consists of DNA and the protein complexes called histones around which genomic DNA is packed. In a given cell, chromatin organization, like methylation, helps to determine whether specific genes can be expressed or not.
###

"Directional DNA Methylation Changes and Complex Intermediate States Accompany Lineage Specificity in the Adult Hematopoietic Compartment" appears in Molecular Cell October 7, 2011. The paper was published online ahead of print and can be accessed at: doi:10.1016/j.molcel.2011.08.026. The authors are: Emily Hodges, Antoine Molaro, Camila O. Dos Santos, Pramod Thekkat, Qiang Song, Philip J. Uren, Jin Park, Jason Butler, Shahin Rafii, W. Richard McCombie, Andrew D. Smith and Gregory J. Hannon.

This research was supported in part by grants from the National Institutes of Health and a kind gift from Kathryn W. Davis. Other supporting funders include: the National Human Genome Research Institute, the Burroughs Wellcome Fund, Massachusetts General Hospital and the Broad Institute.
About CSHL

Founded in 1890, Cold Spring Harbor Laboratory (CSHL) has shaped contemporary biomedical research and education with programs in cancer, neuroscience, plant biology and quantitative biology. CSHL is ranked number one in the world by Thomson Reuters for impact of its research in molecular biology and genetics. The Laboratory has been home to eight Nobel Prize winners. Today, CSHL's multidisciplinary scientific community is more than 400 scientists strong and its Meetings & Courses program hosts more than 8,000 scientists from around the world each year. Tens of thousands more benefit from the research, reviews, and ideas published in journals and books distributed internationally by CSHL Press. The Laboratory's education arm also includes a graduate school and programs for undergraduates as well as middle and high school students and teachers. CSHL is a private, not-for-profit institution on the north shore of Long Island.

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AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.

Research reveals how dynamic changes in methylation can determine cell fate Public release date: 26-Sep-2011
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Contact: Peter Tarr
tarr@cshl.edu
516-367-8455
Cold Spring Harbor Laboratory

Cold Spring Harbor, NY Scientists at Cold Spring Harbor Laboratory (CSHL) and the University of Southern California (USC) have uncovered intriguing new evidence helping to explain one of the ways in which a stem cell's fate can be determined.

The new data show how the "marking" of DNA sequences by groups of methyl molecules a process called methylation can influence the type of cell a stem cell will become. The cellular maturation process, called differentiation, has long been thought to be affected by methylation. Subtle changes in methylation patterns within subsets of a particular cell type have now been observed and closely scrutinized, and they reveal some intriguing mechanisms at work in the process.

A team led by postdoc Dr. Emily Hodges, working in the laboratory of CSHL Professor and HHMI Investigator Gregory Hannon, studied how methylation changes in blood stem cells can affect whether a given stem cell will differentiate into either a myeloid cell or a lymphoid cell. These are the two major lineages of mature blood cells. Sophisticated mathematical analyses of the data were performed under the direction of USC Professor Andrew D. Smith.

The study, which will appear in print October 7 in the journal Molecular Cell, generated some surprising findings that challenge currently held theories about how methylation operates. First, it demonstrated that methylation patterns are more dynamic than they are often thought to be. "It's not a question of methylation being 'on' or 'off' at a given site in the genome," explains Hodges. "We find, instead, an interesting fluctuation of the boundaries of regions that are free of methylation marks. This fact, in turn, can have a profound impact upon cell fate."

Areas lacking methylation, called hypomethylated regions, or HMRs, tend to coincide with so-called CpG islands, sites in the genome where adjacent "Cs" and "G's" cytosine and guanine nucleotides are seen in strings of repeats. These unmethylated regions tend to be ones associated with nearby genes that are capable of being expressed. In contrast, sites in the genome that are methylated are typically not expressed.

The new study, which looks at these areas at high resolution in cells of the different blood cell lineages and in blood stem cells, finds that in many cases, a core portion of the unmethylated region is shared in common, but that adjacent areas, sometimes called "CpG shores" the outlying areas around CpG islands differ markedly in breadth. The CSHL-USC team refines the notion of islands and shores, preferring to describe the narrowing and widening of the "shoreline" as a tidal phenomenon.

"We observed that the boundaries of these unmethylated regions goes in and out, like the tides," says Hodges. "The key question is what drives these changes. We found that the width of these regions depends on the gene that is associated with the region. We showed in blood cells that the variation is lineage-specific."

The team deduced this after making close study of the methylation patterns in genomic regions containing genes known from other research to be expressed specifically in lymphoid cells, but not in myeloid cells, or vice versa. In these cases, all blood cells share a narrow "core" region of hypomethylation; but only in one lineage did the unmethylated region widen a widening that opens the promoter of the "underlying" gene to the cellular machinery initiating gene expression. In other words, the lack of methylation over a wider area enables the underlying gene to be activated only in the specified cell-type, but not in any of the others.

Another striking observation made from this data is the directional preference of this expansion. For example, in the widening of the unmethylated region seen in the case of the lymphoid cell, the direction of the widening was toward the area occupied by the underlying gene, which in this case was a gene encoding a B cell surface marker called CD22.

It has generally been thought that methylation is a stable epigenetic mark and that changes in methylation are unidirectional; and further, that cells become increasingly methylated as they move through the differentiation process toward their mature identity. And in fact, the only known direction of active change is from an unmethylated state to a methylated state.

The new data suggests, however, that dynamic changes in methylation status may be possible. The relevant evidence comes from blood stem cells, which were observed to have methylation patterns that the team describes as "intermediately methylated," seemingly in dynamic equilibria of the two extreme states of "methylated" and "unmethylated."

According to Hodges, this raises the possibility that methylation might in fact be bidirectional, and that there might be an as yet undiscovered, active mechanism that performs de-methylation. No known enzyme has this ability to remove methyl groups from DNA; DNA methyltransferase is the well-known enzyme that catalyzes the addition of methyl groups.

Yet another of the team's unexpected findings concerns the position of HMRs relative to know genic regions. While unmethylated regions tend to be associated with nearby genes that are capable of being expressed, the team found, according to Hodges, "a lot of HMRs located far away from any annotated gene locus." One notable thing about these regions, she says, "is that they were highly enriched for binding sites of specific regulatory molecules that are involved in chromatin organization."

Chromatin consists of DNA and the protein complexes called histones around which genomic DNA is packed. In a given cell, chromatin organization, like methylation, helps to determine whether specific genes can be expressed or not.
###

"Directional DNA Methylation Changes and Complex Intermediate States Accompany Lineage Specificity in the Adult Hematopoietic Compartment" appears in Molecular Cell October 7, 2011. The paper was published online ahead of print and can be accessed at: doi:10.1016/j.molcel.2011.08.026. The authors are: Emily Hodges, Antoine Molaro, Camila O. Dos Santos, Pramod Thekkat, Qiang Song, Philip J. Uren, Jin Park, Jason Butler, Shahin Rafii, W. Richard McCombie, Andrew D. Smith and Gregory J. Hannon.

This research was supported in part by grants from the National Institutes of Health and a kind gift from Kathryn W. Davis. Other supporting funders include: the National Human Genome Research Institute, the Burroughs Wellcome Fund, Massachusetts General Hospital and the Broad Institute.
About CSHL

Founded in 1890, Cold Spring Harbor Laboratory (CSHL) has shaped contemporary biomedical research and education with programs in cancer, neuroscience, plant biology and quantitative biology. CSHL is ranked number one in the world by Thomson Reuters for impact of its research in molecular biology and genetics. The Laboratory has been home to eight Nobel Prize winners. Today, CSHL's multidisciplinary scientific community is more than 400 scientists strong and its Meetings & Courses program hosts more than 8,000 scientists from around the world each year. Tens of thousands more benefit from the research, reviews, and ideas published in journals and books distributed internationally by CSHL Press. The Laboratory's education arm also includes a graduate school and programs for undergraduates as well as middle and high school students and teachers. CSHL is a private, not-for-profit institution on the north shore of Long Island.

[ | E-mail | Share ]
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AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.

Source: http://www.eurekalert.org/pub_releases/2011-09/cshl-rrh092611.php
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Fla. millionaire guilty of murdering wife

ORLANDO, Fla.?? A millionaire developer who lived in the same central Florida neighborhood as Tiger Woods and other celebrities was convicted Saturday of murdering his wife in their mansion.

The six jurors deliberated more than 12 hours over two days before finding Bob Ward guilty of second-degree murder. The two-week trial took place in the same courthouse where the Casey Anthony case was tried this summer.

Ward was stoic as the verdict was read. Before the verdict, he hugged his two college-aged daughters as the women wept.

He faces up to life in prison when he's sentenced in November.

Ward was accused of shooting of his wife, Diane, in September 2009.The slaying happened days before she was to give a deposition in a lawsuit alleging that he blew millions of dollars on big houses and expensive cars while his business failed. Defense attorneys said that Ward's wife was suicidal, and that he was trying to stop her from killing herself. Ward never took the stand in his defense.

Defense attorney Kirk Kirkconnell said he plans to appeal the verdict and request a new trial.

Kirkconnell said jurors may have been more likely to convict Ward as the result of Casey Anthony's trial. The Florida mother was acquitted this summer of killing her 2-year-old daughter in a verdict that surprised followers of the case.

"What happened to Casey Anthony certainly makes it more difficult for any defendant, probably anywhere in the state of Florida, to get a fair trial," Kirkconnell said. "I think there is a widely held belief, or prejudice, based on the Casey Anthony case because people may have felt that that verdict was not the proper verdict."

During closing arguments Thursday, prosecutor Robin Wilkinson used Ward's own words against him by playing a recording of the emergency call to police in which he told a dispatcher five times, "I just shot my wife."

After Saturday's verdict, Wilkinson said she felt sorry for the Wards' two daughters, who had lost a mother and were now losing a father to prison. But she said justice had been served.

"Who wants to believe that their father killed their mother?" Wilkinson said. "I believe Diane Ward got justice today."

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The Wards were under tremendous stress from the bankruptcy of his company and a lawsuit over his business practices. A broken wine glass, a wine stain on the patio and a similar stain on Ward's shirt indicated they had been fighting before Diane Ward died, Wilkinson told jurors during closing arguments.

Kirkconnell told jurors it was against Bob Ward's interests to kill his wife because her death exposed his assets to his creditors.

Bob Ward's company, Land Resource, filed for bankruptcy almost a year before the shooting. He was being sued by an insurance company that accused him of taking more than $20 million from the sale of lots in subdivisions he was building in Tennessee in 2007. The insurance company, which had issued bonds for the subdivisions, said Ward should have used the money to improve the subdivisions, but instead paid off debts and went on a spending spree.

The lawsuit says the Wards used the money to buy their 8,800-square-foot (818-square-meter) mansion in Isleworth, the same neighborhood where Woods had the notorious SUV crash that revealed his adultery and derailed his golf career.

The lawsuit also says the Wards paid off loans totaling nearly $2.5 million for two houses in Georgia, bought a house on the Georgia coast for $750,000 and purchased several vehicles, including a $140,000 Mercedes-Benz sedan. The lawsuit was dismissed last month because of a lack of action by either side.

Ward's behavior after his arrest also raised eyebrows. A jailhouse video of a visit from his daughter and sister-in-law just days after the shooting showed him cracking jokes and laughing. But the Wards' two daughters have supported their father and were in the courtroom during the trial.

Source: http://www.msnbc.msn.com/id/44651948/ns/us_news-crime_and_courts/

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Fishhawk Ranch Real Estate | Homes for sale | Tampa Florida ...

by Richie on September 24, 2011

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Source: http://tampaflhomesearch.com/blog/fishhawk-ranch-real-estate-homes-for-sale-2/

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Monday, September 26, 2011

Libyan forces fight for Gadhafi's hometown Sirte

A pall of smoke covers the city of Tripoli, Libya, as it rises from the military warehouse belonged to the army of Libya's ousted leader Moammar Gadhafi in the port area of the city, Saturday, Sept. 24, 2011. A series of explosions rocked the military warehouse and a huge plume of smoke rose over the harbor on Saturday afternoon, although the cause of the blasts is not immediately known. (AP Photo/Bela Szandelszky)

Revolutionary fighters take cover from heavy artillery, rocket and missile counter-attacks launched by Gadhafi loyalists in Sirte, Libya, Saturday, Sept. 24, 2011. Backed by rockets and heavy artillery, hundreds of Libyan revolutionary fighters pushed into Moammar Gadhafi's hometown from the west Saturday in the first significant assault in about a week. (AP Photo/Gaia Anderson)

People pose for photos with a pre-Gadhafi's flag, as a pall of smoke rises from a military warehouse belonged to the army of Libya's ousted leader Moammar Gadhafi in the port area of Tripoli, Libya Saturday, Sept. 24, 2011. A series of explosions rocked the military warehouse and a huge plume of smoke rose over the harbor on Saturday afternoon, although the cause of the blasts is not immediately known. (AP Photo/Alexandre Meneghini)

Libyans visit the destroyed Bab al-Azizia military barracks and compound of their country's ousted leader Moammar Gadhafi, in the southern suburbs of Tripoli, Libya, Saturday, Sept. 24, 2011. The compound was the main base for the Libyan leader until its capture by the revolutionary fighters on August 23. (AP Photo/Bela Szandelszky)

Mahmood Jibril, chairman of Libya's Transitional National Council executive board, addresses the 66th session of the United Nations General Assembly at U.N. headquarters, Saturday, Sept. 24, 2011. (AP Photo/Mary Altaffer)

SIRTE, Libya (AP) ? With NATO jets roaring overhead, revolutionary forces fought their way into Moammar Gadhafi's hometown Saturday in the first significant push into the stubborn stronghold in about a week.

Libya's new leaders also tried to move on the political front, promising to announce in the coming week a new interim government that it hopes will help unite the country. However, disagreements remain about what the Cabinet should look like.

The National Transitional Council led the rebellion that forced Gadhafi into hiding and has taken over the leadership of the oil-rich North African nation even as it continues to fight forces still loyal to the fugitive leader.

The NTC-appointed prime minister, Mahmoud Jibril, sought support from leaders at the United Nations on Saturday, telling them that "a new Libya is coming to life" as a nation committed to democracy, equality and reintegration into the international community. He said the council was committed to drafting a constitution that would be put to the Libyans for a referendum.

More than a month after seizing Tripoli and effectively ending Gadhafi's rule, revolutionary forces have been unable to rout well-armed Gadhafi loyalists from strongholds in his hometown of Sirte, Bani Walid and some southern enclaves. Taking the cities is key for Libya's new leaders to extend their control over the large desert nation.

Explosions rocked Sirte throughout the day as fighters pushing in on four roads came under heavy fire from loyalist snipers and artillery guns. Along the city's main thoroughfare, they faced close-range gunfights with loyalists hiding in apartment buildings and throwing hand grenades at them from windows.

Moftah Mohammed, 28, said snipers shot two of his friends as they advanced to fire a rocket-propelled grenade on a loyalist truck. When others approached to help the wounded, Gadhafi supporters opened fire and hurled hand grenades, injuring two more.

By evening, however, the fighters had pushed east along the city's main thoroughfare into its urban center, overrunning a TV station and pushing loyalists farther back. NATO warplanes patrolled overhead during the fighting, and revolutionary commanders said airstrikes took out some loyalist tanks, although that could not be confirmed immediately.

Walls along the town's main boulevard were pockmarked from heavy caliber machine-gun rounds, and the charred metal hulks of cars lined the streets in front of shuttered shops, some of which had been torched.

Gaping holes marred the walls of the TV building, and two of the Gadhafi regime's green flags still flew from the roof. Two tanks sat nearby, and rebel trucks with mounted machine guns raced forward while blasting at loyalist positions. In front of a convenience store, a group of men fired a half dozen mortars, yelling "God is great!" after each one flew into the distance.

Most of the fighters came from the western city of Misrata, which saw some of the fiercest fighting in the civil war that erupted after Libyans rose up against Gadhafi in mid-February. For the assault on Sirte, they have used many of the urban battle tactics developed in the defense of their own city, including blocking the road with shipping containers and filling them with sand so they couldn't be moved.

"When we fought in Misrata it was all new to us," said Adnan al-Zredi, 25, a former clothing store clerk who manned an anti-aircraft gun on the back of a truck. "Now we're fine in war. We know exactly what to do."

Some fighters said Gadhafi forces in the city had adopted similar tactics, building a similar barricade of shipping containers and sand elsewhere in the city. "They got some ideas from us," fighter Abdel-Aziz Salim said proudly.

He spoke from an elementary school on the city's edge that had been transformed into a military staging ground. Nearby, fighters pounded huge bullets into ammunition belts and armed rocket-propelled grenades before heading back to the front.

Sirte is the Libyan city most associated with Gadhafi. Revolutionary fighters tried to push into the city last weekend but were driven back in fighting that killed at least 25 and wounded dozens. They pulled back to regroup and let civilians leave the area, although the two sides exchanged fire daily.

In the meantime, more than 1,300 families have left the city, fighters said. A few dozen waiting at a checkpoint outside the city on Saturday described rapidly deteriorating conditions. Many had been clustered in basements, eating once a day and drinking water from nearby wells or water tanks. Some said their children had gotten diarrhea from the water.

Over the last week, fighters said they wouldn't attack until all the city's civilians were out. In the end, they decided to advance Saturday because they feared many families from Misrata that were stuck in the city were in danger, said a brigade commander, Mohammed al-Sugatri.

"There are lots of people from Misrata who are stuck in the city living in basements. They have no food or water and many of their children are sick so we had no choice but to attack," he said.

It remains unclear how many civilians remain in the city and how many of them remain loyal to Gadhafi and his forces.

At a small mosque outside town that has been converted into a field hospital, Dr. Mahmoud Khlef said six revolutionary fighters were killed Saturday and close to 80 wounded, most of them by shrapnel from rocket-propelled grenades.

Members of the National Transitional Council have been struggling to form a new interim government amid political infighting over everything from which cities should be represented and how many Cabinet ministers there should be. That has raised concerns that the former rebels will splinter into rival factions now that they no longer have the ouster of Gadhafi as a common cause.

NTC chief Mustafa Abdul-Jalil, speaking to reporters in Benghazi after attending the U.N. General Assembly in New York, acknowledged differences but said a new government would be named next week to guide the country until formal elections can be held.

"This is the crisis management phase and it should be led by people who are efficient, even if they have to be from the same city, until the liberation of the country and until the constitution is established," he said. "Then they can choose a government that they want."

In the capital, Tripoli, a series of explosions went off at a military storage warehouse on a Libyan naval base near the harbor Saturday afternoon and heavy black smoke poured out of the facility, although no injuries were reported. A revolutionary command spokesman, Abdel-Rahman Busin, said it was an accident caused by either an electrical problem or the improper storage of ammunition.

___

Al-Shalchi was reporting from Tripoli. Associated Press writers Kim Gamel in Tripoli and Rami al-Shaheibi contributed to this report.

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/3d281c11a96b4ad082fe88aa0db04305/Article_2011-09-24-ML-Libya/id-01eba88a8cf7469abc6e4c4d979015e9

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