in the news

Wednesday, September 28, 2011

U.S. on "knife edge" of contraction: Fed economist (Reuters)

SAN ANTONIO (Reuters) ? The U.S. economy is on a "knife edge" between growth and contraction, and if it were a dashboard, it would be flashing "watch out, danger ahead on all gauges," Dallas Federal Reserve Bank's top economist said on Tuesday.

"The economy is moving along at stall speed," Dallas Fed research director Harvey Rosenblum told a forum sponsored by the greater San Antonio Chamber of Commerce. "Unless we start moving a little bit faster, we are at a tipping point where things may not go the right way."

The U.S. jobs engine has lost momentum and could be set for further "backtracking," Meanwhile, he said, there is also a "credible" risk of rising inflation.

"We are in the midst of the Second Great Contraction," Rosenblum said, demonstrating the economy's predicament with a picture of a place on the Appalachian Trail known as "Knife's Edge."

"This patient is still not ready to get out of the hospital, there are still tubes connected to the patient, and the patient is still not responding well to all the medicine."

The grim assessment of the economic outlook came a week after a majority of the Fed's policy-setting panel backed further monetary policy easing to help support a faltering U.S. recovery.

Dallas Fed President Richard Fisher, Rosenblum's boss, was one of three policymakers to dissent. Fisher gave a vigorous defense of his decision at his bank headquarters on Tuesday, saying he believed the move could do more harm than good.

In San Antonio, Rosenblum said that there is a better than even chance the United States will return to more than 2 percent growth, rather than fall into recession.

But at the recent policy-setting Federal Open Market Committee meeting, given a choice between whether the economy will be better than expected or worse than expected in the near future, most participants said it would be worse than expected, according to Rosenblum.

"The takeaway on the national economy is that economic growth has slowed and maybe stalled," he said. "We are on an edge."

(Reporting by Jim Forsyth in San Antonio; Writing by Ann Saphir in Dallas; Editing by James Dalgleish)

Source: http://us.rd.yahoo.com/dailynews/rss/business/*http%3A//news.yahoo.com/s/nm/20110927/bs_nm/us_usa_fed_contraction

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Pankaj Advani joins kids' movement

Bangalore, Sep 28

World billiards champion Pankaj Advani is auctioning his time during the 'Joy of Giving Week' to raise funds for Bangalore based NGO Children's Movement for Civic Awareness (CMCA).

CMCA is an 11-year old volunteer driven, civil society organisation that conducts a national 'civic club' programme in schools across India. Over the past year, CMCA has, through 284 volunteers, reached 19,000 children in six cities, 324 schools and 399 civic clubs.

Speaking about his association with the NGO, Advani said: ?For the democratic set-up to function effectively, it is essential for individuals to be aware of their rights, duties and responsibilities. CMCA works towards educating and encouraging the citizens of tomorrow to make a positive impact on the society that they live in.?

The NGO believes that Advani is its perfect goodwill ambassador, as his success inspires the youth of the nation to believe that with hard work and determination nothing is beyond reach.

Expressing joy at Advani's efforts to help them, CMCA's National Coordinator and Managing Trustee Vrunda Bhaskar, said: ?We are happy that young icons and role models like Pankaj Advani are committing themselves to a very important aspect of India's future as a secular and democratic nation - preparing our young to be active and empowered citizens who know how to stand up for their rights and strengthen our democracy.?

During the 'Joy of Giving Week', fans will have a chance to bid to spend time with Advani and contribute to a good cause.

The bidding for a chance to spend time with Pankaj will begin on eBay Friday and closes Oct 20, and the highest bidder will win a lunch and bowling session for two with the billiards icon and all the proceeds from this will go to CMCA.

Source: http://www.prokerala.com/news/articles/a250103.html

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Adult western birthday party invitations - Adult Birthday Party- Have ...

Adult Birthday Party- Have Fun With Adult Parties

Adult Birthday Party- Have Fun With Adult Parties

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Home Page > News and Society > Adult Birthday Party- Have Fun With Adult Parties

Adult Birthday Party- Have Fun With Adult Parties

Posted: Mar 26, 2010 |Comments: 0
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Celebrating birthday has special significance, as it is the day when we receive cherished blessings from our near ones and dear ones in a grand way which are generally accompanied with hosting adult birthday party so as to feel the utmost excitements and joy as well. If you have an upcoming birthday or you are setting plans so as to celebrate your birthday in a special way which will become a source for, to derive fun and enjoyments for the rest of your life then the foremost thing you can opt for, is to host an birthday party in any clubs, or you can even hire a hall with all basic hospitalities required for, to feel the enthrallments associated with the celebrations. If you are planning to host a party accompanied with liquors then you should opt for any alternatives so as to make your children feel comfortable in such an atmosphere because you cannot host a party without your children. So it is your responsibility to find for any alternatives which will not deteriorate the level of fun and at the same time you are able to derive fun and enjoyments along with your family comprised of children.

During warmer season the parties are generally hosted outside in the open surrounding so as to feel the calm and serenity associated with nature. If your house is not big enough so as to accommodate for a party surrounding then you can opt for any barbeque or party hall which are implied with huge surrounding area so as to feel the excitements accompanied with such adult birthday party in the perfect way. For holding an outdoor party it is your concern to arrange it in accordance to the condition of the weather as rain might ruin your arrangements.

You can opt for any theme so as to decorate the ambience for your birthday celebration. It is of great concern that the party must appear grand and elaborate and it is completely different from those celebrating their kid's birthday or even celebrating someone's birthday that might be 70 or even 80 years of age. Adult party means the ambience should appear special in terms of arrangements and foods as well. You can invent gambling themes to your birthday ideas party celebration.

Owing to the western influence people has now become quite familiar with the casino games which are not only considered to be an important way of making money but also it helps in adding a sophisticated look to the party hosted by you. In order to earn for your friends appreciation for holding a unique party then you should implement this particular theme to your birthday party celebration so as to make it appear even more sophisticated and grand. As birthday are considered to be the most memorable event for each one us and so it is our duty to make it appear special for the people whom you are inviting for to celebrate your birthday accompanied with unique hospitality essential for an adult birthday party.

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It's always fun being a little naughty as you grow older, have fun with some Adult Birthday Ideas , and don't forget to check out some Free birthday invitations to add to the magic.

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Tuesday, September 27, 2011

Research reveals how dynamic changes in methylation can determine cell fate

Public release date: 26-Sep-2011
[ | E-mail |

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Contact: Peter Tarr
tarr@cshl.edu
516-367-8455
Cold Spring Harbor Laboratory

Cold Spring Harbor, NY Scientists at Cold Spring Harbor Laboratory (CSHL) and the University of Southern California (USC) have uncovered intriguing new evidence helping to explain one of the ways in which a stem cell's fate can be determined.

The new data show how the "marking" of DNA sequences by groups of methyl molecules a process called methylation can influence the type of cell a stem cell will become. The cellular maturation process, called differentiation, has long been thought to be affected by methylation. Subtle changes in methylation patterns within subsets of a particular cell type have now been observed and closely scrutinized, and they reveal some intriguing mechanisms at work in the process.

A team led by postdoc Dr. Emily Hodges, working in the laboratory of CSHL Professor and HHMI Investigator Gregory Hannon, studied how methylation changes in blood stem cells can affect whether a given stem cell will differentiate into either a myeloid cell or a lymphoid cell. These are the two major lineages of mature blood cells. Sophisticated mathematical analyses of the data were performed under the direction of USC Professor Andrew D. Smith.

The study, which will appear in print October 7 in the journal Molecular Cell, generated some surprising findings that challenge currently held theories about how methylation operates. First, it demonstrated that methylation patterns are more dynamic than they are often thought to be. "It's not a question of methylation being 'on' or 'off' at a given site in the genome," explains Hodges. "We find, instead, an interesting fluctuation of the boundaries of regions that are free of methylation marks. This fact, in turn, can have a profound impact upon cell fate."

Areas lacking methylation, called hypomethylated regions, or HMRs, tend to coincide with so-called CpG islands, sites in the genome where adjacent "Cs" and "G's" cytosine and guanine nucleotides are seen in strings of repeats. These unmethylated regions tend to be ones associated with nearby genes that are capable of being expressed. In contrast, sites in the genome that are methylated are typically not expressed.

The new study, which looks at these areas at high resolution in cells of the different blood cell lineages and in blood stem cells, finds that in many cases, a core portion of the unmethylated region is shared in common, but that adjacent areas, sometimes called "CpG shores" the outlying areas around CpG islands differ markedly in breadth. The CSHL-USC team refines the notion of islands and shores, preferring to describe the narrowing and widening of the "shoreline" as a tidal phenomenon.

"We observed that the boundaries of these unmethylated regions goes in and out, like the tides," says Hodges. "The key question is what drives these changes. We found that the width of these regions depends on the gene that is associated with the region. We showed in blood cells that the variation is lineage-specific."

The team deduced this after making close study of the methylation patterns in genomic regions containing genes known from other research to be expressed specifically in lymphoid cells, but not in myeloid cells, or vice versa. In these cases, all blood cells share a narrow "core" region of hypomethylation; but only in one lineage did the unmethylated region widen a widening that opens the promoter of the "underlying" gene to the cellular machinery initiating gene expression. In other words, the lack of methylation over a wider area enables the underlying gene to be activated only in the specified cell-type, but not in any of the others.

Another striking observation made from this data is the directional preference of this expansion. For example, in the widening of the unmethylated region seen in the case of the lymphoid cell, the direction of the widening was toward the area occupied by the underlying gene, which in this case was a gene encoding a B cell surface marker called CD22.

It has generally been thought that methylation is a stable epigenetic mark and that changes in methylation are unidirectional; and further, that cells become increasingly methylated as they move through the differentiation process toward their mature identity. And in fact, the only known direction of active change is from an unmethylated state to a methylated state.

The new data suggests, however, that dynamic changes in methylation status may be possible. The relevant evidence comes from blood stem cells, which were observed to have methylation patterns that the team describes as "intermediately methylated," seemingly in dynamic equilibria of the two extreme states of "methylated" and "unmethylated."

According to Hodges, this raises the possibility that methylation might in fact be bidirectional, and that there might be an as yet undiscovered, active mechanism that performs de-methylation. No known enzyme has this ability to remove methyl groups from DNA; DNA methyltransferase is the well-known enzyme that catalyzes the addition of methyl groups.

Yet another of the team's unexpected findings concerns the position of HMRs relative to know genic regions. While unmethylated regions tend to be associated with nearby genes that are capable of being expressed, the team found, according to Hodges, "a lot of HMRs located far away from any annotated gene locus." One notable thing about these regions, she says, "is that they were highly enriched for binding sites of specific regulatory molecules that are involved in chromatin organization."

Chromatin consists of DNA and the protein complexes called histones around which genomic DNA is packed. In a given cell, chromatin organization, like methylation, helps to determine whether specific genes can be expressed or not.
###

"Directional DNA Methylation Changes and Complex Intermediate States Accompany Lineage Specificity in the Adult Hematopoietic Compartment" appears in Molecular Cell October 7, 2011. The paper was published online ahead of print and can be accessed at: doi:10.1016/j.molcel.2011.08.026. The authors are: Emily Hodges, Antoine Molaro, Camila O. Dos Santos, Pramod Thekkat, Qiang Song, Philip J. Uren, Jin Park, Jason Butler, Shahin Rafii, W. Richard McCombie, Andrew D. Smith and Gregory J. Hannon.

This research was supported in part by grants from the National Institutes of Health and a kind gift from Kathryn W. Davis. Other supporting funders include: the National Human Genome Research Institute, the Burroughs Wellcome Fund, Massachusetts General Hospital and the Broad Institute.
About CSHL

Founded in 1890, Cold Spring Harbor Laboratory (CSHL) has shaped contemporary biomedical research and education with programs in cancer, neuroscience, plant biology and quantitative biology. CSHL is ranked number one in the world by Thomson Reuters for impact of its research in molecular biology and genetics. The Laboratory has been home to eight Nobel Prize winners. Today, CSHL's multidisciplinary scientific community is more than 400 scientists strong and its Meetings & Courses program hosts more than 8,000 scientists from around the world each year. Tens of thousands more benefit from the research, reviews, and ideas published in journals and books distributed internationally by CSHL Press. The Laboratory's education arm also includes a graduate school and programs for undergraduates as well as middle and high school students and teachers. CSHL is a private, not-for-profit institution on the north shore of Long Island.

[ | E-mail | Share ]
?

AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.

Research reveals how dynamic changes in methylation can determine cell fate Public release date: 26-Sep-2011
[ | E-mail | Share ]
Contact: Peter Tarr
tarr@cshl.edu
516-367-8455
Cold Spring Harbor Laboratory

Cold Spring Harbor, NY Scientists at Cold Spring Harbor Laboratory (CSHL) and the University of Southern California (USC) have uncovered intriguing new evidence helping to explain one of the ways in which a stem cell's fate can be determined.

The new data show how the "marking" of DNA sequences by groups of methyl molecules a process called methylation can influence the type of cell a stem cell will become. The cellular maturation process, called differentiation, has long been thought to be affected by methylation. Subtle changes in methylation patterns within subsets of a particular cell type have now been observed and closely scrutinized, and they reveal some intriguing mechanisms at work in the process.

A team led by postdoc Dr. Emily Hodges, working in the laboratory of CSHL Professor and HHMI Investigator Gregory Hannon, studied how methylation changes in blood stem cells can affect whether a given stem cell will differentiate into either a myeloid cell or a lymphoid cell. These are the two major lineages of mature blood cells. Sophisticated mathematical analyses of the data were performed under the direction of USC Professor Andrew D. Smith.

The study, which will appear in print October 7 in the journal Molecular Cell, generated some surprising findings that challenge currently held theories about how methylation operates. First, it demonstrated that methylation patterns are more dynamic than they are often thought to be. "It's not a question of methylation being 'on' or 'off' at a given site in the genome," explains Hodges. "We find, instead, an interesting fluctuation of the boundaries of regions that are free of methylation marks. This fact, in turn, can have a profound impact upon cell fate."

Areas lacking methylation, called hypomethylated regions, or HMRs, tend to coincide with so-called CpG islands, sites in the genome where adjacent "Cs" and "G's" cytosine and guanine nucleotides are seen in strings of repeats. These unmethylated regions tend to be ones associated with nearby genes that are capable of being expressed. In contrast, sites in the genome that are methylated are typically not expressed.

The new study, which looks at these areas at high resolution in cells of the different blood cell lineages and in blood stem cells, finds that in many cases, a core portion of the unmethylated region is shared in common, but that adjacent areas, sometimes called "CpG shores" the outlying areas around CpG islands differ markedly in breadth. The CSHL-USC team refines the notion of islands and shores, preferring to describe the narrowing and widening of the "shoreline" as a tidal phenomenon.

"We observed that the boundaries of these unmethylated regions goes in and out, like the tides," says Hodges. "The key question is what drives these changes. We found that the width of these regions depends on the gene that is associated with the region. We showed in blood cells that the variation is lineage-specific."

The team deduced this after making close study of the methylation patterns in genomic regions containing genes known from other research to be expressed specifically in lymphoid cells, but not in myeloid cells, or vice versa. In these cases, all blood cells share a narrow "core" region of hypomethylation; but only in one lineage did the unmethylated region widen a widening that opens the promoter of the "underlying" gene to the cellular machinery initiating gene expression. In other words, the lack of methylation over a wider area enables the underlying gene to be activated only in the specified cell-type, but not in any of the others.

Another striking observation made from this data is the directional preference of this expansion. For example, in the widening of the unmethylated region seen in the case of the lymphoid cell, the direction of the widening was toward the area occupied by the underlying gene, which in this case was a gene encoding a B cell surface marker called CD22.

It has generally been thought that methylation is a stable epigenetic mark and that changes in methylation are unidirectional; and further, that cells become increasingly methylated as they move through the differentiation process toward their mature identity. And in fact, the only known direction of active change is from an unmethylated state to a methylated state.

The new data suggests, however, that dynamic changes in methylation status may be possible. The relevant evidence comes from blood stem cells, which were observed to have methylation patterns that the team describes as "intermediately methylated," seemingly in dynamic equilibria of the two extreme states of "methylated" and "unmethylated."

According to Hodges, this raises the possibility that methylation might in fact be bidirectional, and that there might be an as yet undiscovered, active mechanism that performs de-methylation. No known enzyme has this ability to remove methyl groups from DNA; DNA methyltransferase is the well-known enzyme that catalyzes the addition of methyl groups.

Yet another of the team's unexpected findings concerns the position of HMRs relative to know genic regions. While unmethylated regions tend to be associated with nearby genes that are capable of being expressed, the team found, according to Hodges, "a lot of HMRs located far away from any annotated gene locus." One notable thing about these regions, she says, "is that they were highly enriched for binding sites of specific regulatory molecules that are involved in chromatin organization."

Chromatin consists of DNA and the protein complexes called histones around which genomic DNA is packed. In a given cell, chromatin organization, like methylation, helps to determine whether specific genes can be expressed or not.
###

"Directional DNA Methylation Changes and Complex Intermediate States Accompany Lineage Specificity in the Adult Hematopoietic Compartment" appears in Molecular Cell October 7, 2011. The paper was published online ahead of print and can be accessed at: doi:10.1016/j.molcel.2011.08.026. The authors are: Emily Hodges, Antoine Molaro, Camila O. Dos Santos, Pramod Thekkat, Qiang Song, Philip J. Uren, Jin Park, Jason Butler, Shahin Rafii, W. Richard McCombie, Andrew D. Smith and Gregory J. Hannon.

This research was supported in part by grants from the National Institutes of Health and a kind gift from Kathryn W. Davis. Other supporting funders include: the National Human Genome Research Institute, the Burroughs Wellcome Fund, Massachusetts General Hospital and the Broad Institute.
About CSHL

Founded in 1890, Cold Spring Harbor Laboratory (CSHL) has shaped contemporary biomedical research and education with programs in cancer, neuroscience, plant biology and quantitative biology. CSHL is ranked number one in the world by Thomson Reuters for impact of its research in molecular biology and genetics. The Laboratory has been home to eight Nobel Prize winners. Today, CSHL's multidisciplinary scientific community is more than 400 scientists strong and its Meetings & Courses program hosts more than 8,000 scientists from around the world each year. Tens of thousands more benefit from the research, reviews, and ideas published in journals and books distributed internationally by CSHL Press. The Laboratory's education arm also includes a graduate school and programs for undergraduates as well as middle and high school students and teachers. CSHL is a private, not-for-profit institution on the north shore of Long Island.

[ | E-mail | Share ]
?

AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.

Source: http://www.eurekalert.org/pub_releases/2011-09/cshl-rrh092611.php
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Fla. millionaire guilty of murdering wife

ORLANDO, Fla.?? A millionaire developer who lived in the same central Florida neighborhood as Tiger Woods and other celebrities was convicted Saturday of murdering his wife in their mansion.

The six jurors deliberated more than 12 hours over two days before finding Bob Ward guilty of second-degree murder. The two-week trial took place in the same courthouse where the Casey Anthony case was tried this summer.

Ward was stoic as the verdict was read. Before the verdict, he hugged his two college-aged daughters as the women wept.

He faces up to life in prison when he's sentenced in November.

Ward was accused of shooting of his wife, Diane, in September 2009.The slaying happened days before she was to give a deposition in a lawsuit alleging that he blew millions of dollars on big houses and expensive cars while his business failed. Defense attorneys said that Ward's wife was suicidal, and that he was trying to stop her from killing herself. Ward never took the stand in his defense.

Defense attorney Kirk Kirkconnell said he plans to appeal the verdict and request a new trial.

Kirkconnell said jurors may have been more likely to convict Ward as the result of Casey Anthony's trial. The Florida mother was acquitted this summer of killing her 2-year-old daughter in a verdict that surprised followers of the case.

"What happened to Casey Anthony certainly makes it more difficult for any defendant, probably anywhere in the state of Florida, to get a fair trial," Kirkconnell said. "I think there is a widely held belief, or prejudice, based on the Casey Anthony case because people may have felt that that verdict was not the proper verdict."

During closing arguments Thursday, prosecutor Robin Wilkinson used Ward's own words against him by playing a recording of the emergency call to police in which he told a dispatcher five times, "I just shot my wife."

After Saturday's verdict, Wilkinson said she felt sorry for the Wards' two daughters, who had lost a mother and were now losing a father to prison. But she said justice had been served.

"Who wants to believe that their father killed their mother?" Wilkinson said. "I believe Diane Ward got justice today."

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The Wards were under tremendous stress from the bankruptcy of his company and a lawsuit over his business practices. A broken wine glass, a wine stain on the patio and a similar stain on Ward's shirt indicated they had been fighting before Diane Ward died, Wilkinson told jurors during closing arguments.

Kirkconnell told jurors it was against Bob Ward's interests to kill his wife because her death exposed his assets to his creditors.

Bob Ward's company, Land Resource, filed for bankruptcy almost a year before the shooting. He was being sued by an insurance company that accused him of taking more than $20 million from the sale of lots in subdivisions he was building in Tennessee in 2007. The insurance company, which had issued bonds for the subdivisions, said Ward should have used the money to improve the subdivisions, but instead paid off debts and went on a spending spree.

The lawsuit says the Wards used the money to buy their 8,800-square-foot (818-square-meter) mansion in Isleworth, the same neighborhood where Woods had the notorious SUV crash that revealed his adultery and derailed his golf career.

The lawsuit also says the Wards paid off loans totaling nearly $2.5 million for two houses in Georgia, bought a house on the Georgia coast for $750,000 and purchased several vehicles, including a $140,000 Mercedes-Benz sedan. The lawsuit was dismissed last month because of a lack of action by either side.

Ward's behavior after his arrest also raised eyebrows. A jailhouse video of a visit from his daughter and sister-in-law just days after the shooting showed him cracking jokes and laughing. But the Wards' two daughters have supported their father and were in the courtroom during the trial.

Source: http://www.msnbc.msn.com/id/44651948/ns/us_news-crime_and_courts/

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Fishhawk Ranch Real Estate | Homes for sale | Tampa Florida ...

by Richie on September 24, 2011

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Monday, September 26, 2011

Libyan forces fight for Gadhafi's hometown Sirte

A pall of smoke covers the city of Tripoli, Libya, as it rises from the military warehouse belonged to the army of Libya's ousted leader Moammar Gadhafi in the port area of the city, Saturday, Sept. 24, 2011. A series of explosions rocked the military warehouse and a huge plume of smoke rose over the harbor on Saturday afternoon, although the cause of the blasts is not immediately known. (AP Photo/Bela Szandelszky)

Revolutionary fighters take cover from heavy artillery, rocket and missile counter-attacks launched by Gadhafi loyalists in Sirte, Libya, Saturday, Sept. 24, 2011. Backed by rockets and heavy artillery, hundreds of Libyan revolutionary fighters pushed into Moammar Gadhafi's hometown from the west Saturday in the first significant assault in about a week. (AP Photo/Gaia Anderson)

People pose for photos with a pre-Gadhafi's flag, as a pall of smoke rises from a military warehouse belonged to the army of Libya's ousted leader Moammar Gadhafi in the port area of Tripoli, Libya Saturday, Sept. 24, 2011. A series of explosions rocked the military warehouse and a huge plume of smoke rose over the harbor on Saturday afternoon, although the cause of the blasts is not immediately known. (AP Photo/Alexandre Meneghini)

Libyans visit the destroyed Bab al-Azizia military barracks and compound of their country's ousted leader Moammar Gadhafi, in the southern suburbs of Tripoli, Libya, Saturday, Sept. 24, 2011. The compound was the main base for the Libyan leader until its capture by the revolutionary fighters on August 23. (AP Photo/Bela Szandelszky)

Mahmood Jibril, chairman of Libya's Transitional National Council executive board, addresses the 66th session of the United Nations General Assembly at U.N. headquarters, Saturday, Sept. 24, 2011. (AP Photo/Mary Altaffer)

SIRTE, Libya (AP) ? With NATO jets roaring overhead, revolutionary forces fought their way into Moammar Gadhafi's hometown Saturday in the first significant push into the stubborn stronghold in about a week.

Libya's new leaders also tried to move on the political front, promising to announce in the coming week a new interim government that it hopes will help unite the country. However, disagreements remain about what the Cabinet should look like.

The National Transitional Council led the rebellion that forced Gadhafi into hiding and has taken over the leadership of the oil-rich North African nation even as it continues to fight forces still loyal to the fugitive leader.

The NTC-appointed prime minister, Mahmoud Jibril, sought support from leaders at the United Nations on Saturday, telling them that "a new Libya is coming to life" as a nation committed to democracy, equality and reintegration into the international community. He said the council was committed to drafting a constitution that would be put to the Libyans for a referendum.

More than a month after seizing Tripoli and effectively ending Gadhafi's rule, revolutionary forces have been unable to rout well-armed Gadhafi loyalists from strongholds in his hometown of Sirte, Bani Walid and some southern enclaves. Taking the cities is key for Libya's new leaders to extend their control over the large desert nation.

Explosions rocked Sirte throughout the day as fighters pushing in on four roads came under heavy fire from loyalist snipers and artillery guns. Along the city's main thoroughfare, they faced close-range gunfights with loyalists hiding in apartment buildings and throwing hand grenades at them from windows.

Moftah Mohammed, 28, said snipers shot two of his friends as they advanced to fire a rocket-propelled grenade on a loyalist truck. When others approached to help the wounded, Gadhafi supporters opened fire and hurled hand grenades, injuring two more.

By evening, however, the fighters had pushed east along the city's main thoroughfare into its urban center, overrunning a TV station and pushing loyalists farther back. NATO warplanes patrolled overhead during the fighting, and revolutionary commanders said airstrikes took out some loyalist tanks, although that could not be confirmed immediately.

Walls along the town's main boulevard were pockmarked from heavy caliber machine-gun rounds, and the charred metal hulks of cars lined the streets in front of shuttered shops, some of which had been torched.

Gaping holes marred the walls of the TV building, and two of the Gadhafi regime's green flags still flew from the roof. Two tanks sat nearby, and rebel trucks with mounted machine guns raced forward while blasting at loyalist positions. In front of a convenience store, a group of men fired a half dozen mortars, yelling "God is great!" after each one flew into the distance.

Most of the fighters came from the western city of Misrata, which saw some of the fiercest fighting in the civil war that erupted after Libyans rose up against Gadhafi in mid-February. For the assault on Sirte, they have used many of the urban battle tactics developed in the defense of their own city, including blocking the road with shipping containers and filling them with sand so they couldn't be moved.

"When we fought in Misrata it was all new to us," said Adnan al-Zredi, 25, a former clothing store clerk who manned an anti-aircraft gun on the back of a truck. "Now we're fine in war. We know exactly what to do."

Some fighters said Gadhafi forces in the city had adopted similar tactics, building a similar barricade of shipping containers and sand elsewhere in the city. "They got some ideas from us," fighter Abdel-Aziz Salim said proudly.

He spoke from an elementary school on the city's edge that had been transformed into a military staging ground. Nearby, fighters pounded huge bullets into ammunition belts and armed rocket-propelled grenades before heading back to the front.

Sirte is the Libyan city most associated with Gadhafi. Revolutionary fighters tried to push into the city last weekend but were driven back in fighting that killed at least 25 and wounded dozens. They pulled back to regroup and let civilians leave the area, although the two sides exchanged fire daily.

In the meantime, more than 1,300 families have left the city, fighters said. A few dozen waiting at a checkpoint outside the city on Saturday described rapidly deteriorating conditions. Many had been clustered in basements, eating once a day and drinking water from nearby wells or water tanks. Some said their children had gotten diarrhea from the water.

Over the last week, fighters said they wouldn't attack until all the city's civilians were out. In the end, they decided to advance Saturday because they feared many families from Misrata that were stuck in the city were in danger, said a brigade commander, Mohammed al-Sugatri.

"There are lots of people from Misrata who are stuck in the city living in basements. They have no food or water and many of their children are sick so we had no choice but to attack," he said.

It remains unclear how many civilians remain in the city and how many of them remain loyal to Gadhafi and his forces.

At a small mosque outside town that has been converted into a field hospital, Dr. Mahmoud Khlef said six revolutionary fighters were killed Saturday and close to 80 wounded, most of them by shrapnel from rocket-propelled grenades.

Members of the National Transitional Council have been struggling to form a new interim government amid political infighting over everything from which cities should be represented and how many Cabinet ministers there should be. That has raised concerns that the former rebels will splinter into rival factions now that they no longer have the ouster of Gadhafi as a common cause.

NTC chief Mustafa Abdul-Jalil, speaking to reporters in Benghazi after attending the U.N. General Assembly in New York, acknowledged differences but said a new government would be named next week to guide the country until formal elections can be held.

"This is the crisis management phase and it should be led by people who are efficient, even if they have to be from the same city, until the liberation of the country and until the constitution is established," he said. "Then they can choose a government that they want."

In the capital, Tripoli, a series of explosions went off at a military storage warehouse on a Libyan naval base near the harbor Saturday afternoon and heavy black smoke poured out of the facility, although no injuries were reported. A revolutionary command spokesman, Abdel-Rahman Busin, said it was an accident caused by either an electrical problem or the improper storage of ammunition.

___

Al-Shalchi was reporting from Tripoli. Associated Press writers Kim Gamel in Tripoli and Rami al-Shaheibi contributed to this report.

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/3d281c11a96b4ad082fe88aa0db04305/Article_2011-09-24-ML-Libya/id-01eba88a8cf7469abc6e4c4d979015e9

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